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pna  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pna
    Pna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AS-2074-1/Anti-Peanut+Agglutinin+(PNA)%2C+Unconjugated/pmc12165800-232-61-63
    Average 93 stars, based on 169 article reviews
    pna - by Bioz Stars, 2026-11
    93/100 stars

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    Staining:

    Article Title: M cells targeted H. pylori antigen SAM-FAdE displayed on bacterium-like particles induce protective immunity
    Article Snippet: .. Stomach tissues from mice orally administered PBS, BLPs, SAM-FAdE, and BLPs-SAM-FAdE were embedded, sectioned into 10 μm thick slices, fixed, and blocked, then stained with the following antibodies: BV510-anti mouse CD4 (BD Biosciences), FITC-anti mouse B220 (Invitrogen), and Rhodamine-PNA (Vector). .. After mounting, observations were made using a confocal microscope (ZEISS, Germany).

    Article Title: Vitamin E supplementation prevents ferroptosis in round spermatids of aged mice
    Article Snippet: .. The slides were then incubated with primary antibodies diluted in blocking solution overnight at 4°C, washed again with TBST and incubated for 1 hour at RT with 4’, 6-diamino-2-phenylindole (DAPI, 1:1000, Sigma) as well as secondary antibodies (1:500, Anti-mouse alexa 488 or anti-rabbit Cy3, Jackson ImmunoResearch) or peanut agglutinin (PNA-Cy5, 1:2000, Vector Laboratories) when staining the acrosome for round spermatid identification. .. The sections were washed again with TBST and mounted with Prolong Glass Antifade Mountant (Thermofisher).

    Article Title: M cells targeted H. pylori antigen SAM-FAdE displayed on bacterium-like particles induce protective immunity.
    Article Snippet: .. Stomach tissues from mice orally administered PBS, BLPs, SAMFAdE, and BLPs-SAM-FAdE were embedded, sectioned into 10 μm thick slices, fixed, and blocked, then stained with the following antibodies: BV510-anti mouse CD4 (BD Biosciences), FITC-anti mouse B220 (Invitrogen), and Rhodamine-PNA (Vector). .. After mounting, observations were made using a confocal microscope (ZEISS, Germany).

    Incubation:

    Article Title: β-catenin-inhibited Sumoylation modification of LKB1 and fatty acid metabolism is critical in renal fibrosis
    Article Snippet: .. After permeabilizing with 0.2% Triton X-100 for 10 min, then blocked slides with 10% normal donkey serum for 1 h. Slides were incubated with primary antibodies against anti-β-catenin (BD Biosciences, Cat. 610154, 1:150), anti-Sumo3 (Abcam, Cat.ab203570, 1:50), anti-ADRP (Abcam, Cat. ab52356, 1:50), anti-fibronectin (Sigma, Cat. F3648; 1:100), anti-p-AMPKα (Cell Signaling Technology, Cat. #2535s, 1:50) and anti-CPT1A (Abcam, Cat. ab128568, 1:50), anti-Lotus Tetragonolobus Lectin (LTL) (VECTOR Laboratories, Cat. FL-1321,1:400), anti-Peanut Agglutinin (PNA) (VECTOR Laboratories, Cat. FL-107, 1:400), anti-Dolichos Biflorus Agglutinin (DBA) (VECTOR Laboratories, Cat. #FL1031, 1:400), and anti-E-cadherin (Cell Signaling Technology, Cat. #3195s, 1:1000). .. After washing with TBS-T, slides were incubated with Cy2 or Cy3-conjugated donkey anti-mouse or anti-rabbit IgG (Jackson Immuno-Research Laboratories, West Grove, PA, USA).

    Article Title: Vitamin E supplementation prevents ferroptosis in round spermatids of aged mice
    Article Snippet: .. The slides were then incubated with primary antibodies diluted in blocking solution overnight at 4°C, washed again with TBST and incubated for 1 hour at RT with 4’, 6-diamino-2-phenylindole (DAPI, 1:1000, Sigma) as well as secondary antibodies (1:500, Anti-mouse alexa 488 or anti-rabbit Cy3, Jackson ImmunoResearch) or peanut agglutinin (PNA-Cy5, 1:2000, Vector Laboratories) when staining the acrosome for round spermatid identification. .. The sections were washed again with TBST and mounted with Prolong Glass Antifade Mountant (Thermofisher).

    Article Title: Spsb3 is not essential for spermatogenesis and male fertility in mice
    Article Snippet: .. Briefly, tissue sections were incubated overnight (12-16 hours) at 4°C with the following primary antibodies: Anti-LIN28 (1:200, Abcam), anti-γH2AX (1:1000, Abcam), PNA (1:500, Vector), anti-SOX9 (1:200, Millipore) and anti-HSD-3β (1:500, Santa Cruz Biotechnology). .. Following three washes with PBS at room temperature, sections were incubated with AlexaFluor-conjugated secondary antibodies (Thermo Scientific, Waltham, USA) at 37°C for 1 hour.

    Blocking Assay:

    Article Title: Vitamin E supplementation prevents ferroptosis in round spermatids of aged mice
    Article Snippet: .. The slides were then incubated with primary antibodies diluted in blocking solution overnight at 4°C, washed again with TBST and incubated for 1 hour at RT with 4’, 6-diamino-2-phenylindole (DAPI, 1:1000, Sigma) as well as secondary antibodies (1:500, Anti-mouse alexa 488 or anti-rabbit Cy3, Jackson ImmunoResearch) or peanut agglutinin (PNA-Cy5, 1:2000, Vector Laboratories) when staining the acrosome for round spermatid identification. .. The sections were washed again with TBST and mounted with Prolong Glass Antifade Mountant (Thermofisher).

    Immunohistochemical staining:

    Article Title: SUPPLEMENTARY INFORMATION KMT2D acetylation by CREBBP reveals a cooperative functional interaction in normal and malignant germinal center B cells
    Article Snippet: Histological analysis of mouse lymphoid organs was performed on 3μm-thick FFPE tissue sections, stained with Hematoxylin & Eosin (Thermo Scientific) according to standard procedures. .. The following primary antibodies were used for immunohistochemical analysis: anti-Bcl6 (1:300) (N3, rabbit polyclonal, Santa Cruz Biotechnology); biotin- conjugated anti-PNA (1:200) (Vector Laboratories); biotin-conjugated anti-B220 (1:400) (RA3-6B2, rat monoclonal, Pharmingen 553086) and anti-CD3 (1:800) (SP7, rabbit monoclonal, NeoMarkers RM9107)(Dataset S05b). .. A polymer-enhanced HRP-conjugated secondary antibody (for CD3 and BCL6; EnVision system, Agilent-Dako) or alkaline phosphatase (AP)-conjugated streptavidin (for B220 detection; Vector Laboratories) were then used prior to detection with either AEC substrate (HRP-conjugates, red color)(Sigma- Aldrich) or NBT/BCIP substrate (AP-conjugates, blue color)(Roche).



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